NR AFIQ
AU Herrmann,L.M.; Baszler,T.V.; Knowles,D.P.; Cheevers,W.P.
TI PrPsc is not detected in peripheral blood leukocytes of scrapie-infected sheep: determining the limit of sensitivity by immunohistochemistry.
QU Clinical and Diagnostic Laboratory Immunology 2002 Mar; 9(2): 499-502
PT journal article
AB Peripheral blood leukocytes (PBLs) from scrapie-infected sheep were evaluated for the presence of PrPsc by using dissociated retropharyngeal lymph node (DRLN) cells and immunohistochemistry (IHC). PrPsc-positive cells were detected in 2.05% +/- 0.28% of 3 x 10(6) DRLN cells, but were not detected in 3 x 10(6) PBLs from scrapie-infected sheep. Titration of DRLN cells mixed with PBLs showed that IHC detects a minimum of 0.00205% or 60 PrPsc-positive cells in 3 x 10(6) PBLs.
MH Animal; Antibodies, Monoclonal/diagnostic use; Immunohistochemistry/*standards; Leukocytes/*chemistry; PrPsc Proteins/*analysis/immunology; Scrapie/*diagnosis; Sensitivity and Specificity; Sheep; Support, U.S. Gov't, Non-P.H.S.
AD USDA Agricultural Research Service ADRU, Pullman, Washington 99164, USA. lherrman@vetmed.wsu.edu
SP englisch
PO USA