NR AFCT
AU Hardt,M.; Baron,T.G.M.; Groschup,M.H.
TI A comparative study of immunohistochemical methods for detecting abnormal prion protein with monoclonal and polyclonal antibodies
QU Journal of Comparative Pathology 2000 Jan; 122(1): 43-53
PT journal article
AB Transmissible spongiform encephalopathies are associated with the accumulation of abnormal prion protein (PrPsc) in the central nervous system which can be detected immunohistochemically. Using a monoclonal antibody (L42) to an epitope on the first alpha-helix of ruminant PrP, we compared previously reported immunohistochemical antigen unmasking and "visualization" systems. In addition, a variety of polyclonal and monoclonal antibodies to other epitopes on ruminant PrP were assessed. Antigen unmasking by hydrated autoclaving and proteinase K treatments, and antigen detection with L42 and an avidin-biotin complex system, enabled intra- and extra-neuronal PrPscto be demonstrated in scrapie-affected sheep carrying three different PrP alleles, as well as in cases of bovine spongiform encephalopathy.
MH Amino Acid Sequence; Animal; Antibodies, Monoclonal/*immunology; Brain/*immunology/pathology; Cattle; Comparative Study; Encephalopathy, Bovine Spongiform/*immunology/pathology; Epitopes/immunology; Female; Hamsters; Immunoenzyme Techniques/*veterinary; Mice; Molecular Sequence Data; PrPsc Proteins/*analysis/immunology; Rabbits; Scrapie/*immunology/pathology; Sheep; Sheep Diseases/*immunology/pathology; Species Specificity; Support, Non-U.S. Gov't
AD Federal Research Centre for Virus Diseases of Animals, Tübingen, Germany.
SP englisch
PO England