NR AEYM
AU Guiroy,D.C.; Williams,E.S.; Song,K.J.; Yanagihara,R.; Gajdusek,D.C.
TI Fibrils in brain of Rocky Mountain elk with chronic wasting disease contain scrapie amyloid
QU Acta Neuropathologica 1993; 86(1): 77-80
PT journal article
AB Chronic wasting disease (CWD), a progressive, fatal neurological disorder of captive mule deer and Rocky Mountain elk, is characterized neuropathologically by spongiform change in the neuropil, intraneuronal vacuolation and astrocytic hypertrophy and hyperplasia. Recently, scrapie amyloid-immunoreactive plaques have been demonstrated in brain tissues of CWD-affected captive mule deer, Rocky Mountain elk and hybrids of captive mule deer and white-tailed deer. We now report on the presence of abnormal fibrils isolated from brain tissues of Rocky Mountain elk using negative-stain electron microscopy. These fibrils resemble those found in scrapie-infected hamster brain. Furthermore, protein bands with relative molecular masses of 26 to 30 kilodaltons were shown to be immunoreactive to antibodies raised against scrapie amyloid by Western immunoblotting. Immuno-dot blot showed similar reactivity. Our data support the clinical and pathological diagnosis of the disease and provide further evidence that CWD belongs to the subacute spongiform encephalopathies.
MH Amyloid/*metabolism; Animal; Blotting, Western; Brain/metabolism/pathology; *Deer; Electrophoresis, Polyacrylamide Gel; Hamsters; Immunoblotting; Immunosorbent Techniques; Mesocricetus; Microscopy, Electron; Nerve Tissue Proteins/immunology; Neurofibrils/*metabolism/ultrastructure; Prion Diseases/metabolism/pathology/*veterinary; Scrapie/metabolism/*pathology; Support, Non-U.S. Gov't
AD Laboratory of Central Nervous System Studies, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, MD 20892.
SP englisch
PO Deutschland