NR AEVU
AU Green,A.J.E.; Ramljak,S.; Müller,W.E.G.; Knight,R.S.G.; Schröder,H.C.
TI 14-3-3 in the cerebrospinal fluid of patients with variant and sporadic Creutzfeldt-Jakob disease measured using capture assay able to detect low levels of 14-3-3 protein
QU Neuroscience Letters 2002 May 10; 324(1): 57-60
PT journal article
AB A protein capture assay was used to measure 14-3-3 (-isoform) in the cerebrospinal fluid (CSF) of patients with either variant or sporadic Creutzfeldt-Jakob disease (CJD). The results were compared with those obtained using Western blotting. Elevated levels of 14-3-3 were found in 58% of variant CJD (vCJD) patients and 82% of sporadic CJD (spCJD) patients using the protein capture assay. Using a Western blotting technique, the presence of CSF 14-3-3 was detected in 58% of vCJD patients and in 89% of spCJD patients. When the results from the protein capture assay and the Western blot were combined, 14-3-3 was detected in 77% of vCJD patients and in 91% of spCJD patients. These results suggest that although analysis of CSF 14-3-3 is not as useful in vCJD as it is in the sporadic form of the disease, a combination of these two techniques results in increased sensitivity of 14-3-3 for the diagnosis of vCJD.
MH Aged; Amino Acid Motifs/physiology; Biological Assay/methods; Biological Markers; Blotting, Western; Brain/*metabolism/pathology/physiopathology; Comparative Study; Creutzfeldt-Jakob Syndrome/*cerebrospinal fluid/*diagnosis/physiopathology; Diagnosis, Differential; Female; Human; Male; Middle Age; Neurons/*metabolism/pathology; Predictive Value of Tests; Reproducibility of Results; Statistics; Support, Non-U.S. Gov't; Tyrosine 3-Monooxygenase/*cerebrospinal fluid; Up-Regulation/*physiology
AD Alison J. E. Green (alison.green@ed.ac.uk), Richard S. G. Knight, National CJD Surveillance Unit, University of Edinburgh, Western General Hospital, Crewe Road, EH4 2XU, UK; Sanja Ramljak, Werner E. G. Müller, Heinz C. Schröder, Institut für Physiologische Chemie, Universität Mainz, Duesbergweg 6, 55099 Mainz, Germany
SP englisch
PO Irland