NR AESJ
AU Gomi,H.; Ikeda,T.; Kunieda,T.; Itohara,S.; Prusiner,S.B.; Yamanouchi,K.
TI Prion protein (PrP) is not involved in the pathogenesis of spongiform encephalopathy in zitter rats
QU Neuroscience Letters 1994 Jan 31; 166(2): 171-4
PT journal article
AB In order to elucidate the relationship between the prion protein (PrP) structure and the development of spongiform encephalopathy in zitter rats, we analyzed the nucleotide sequences and restriction fragment length variation (RFLV) of the Prn gene encoding PrP in zitter rats and inbred SD/J rats as a control. Prn genes from two strains had identical nucleotide sequences in their coding sequences. Obvious RFLV on the locus was not detected in zitter rats by a Southern blot hybridization. Consistently, zitter rat brains express the normal cellular PrP (PrPc), but do not accumulate the protease-resistant modified isoform (PrPsc). These results indicate that PrP is not involved in the pathogenesis of spongiform encephalopathy in zitter rats.
IN Bei Ratten gibt es eine erbliche schwammförmige Enzephalopathie, deren Prionproteingen keine Mutation in der kodierenden Region aufweist. Außerdem wurde in den Gehirnen dieser Zitterratten keine Akkumulation anormaler Prionproteine gefunden. Demnach scheint das Prionprotein bei dieser spongiformen Enzephalopathie zumindest keine ursächliche Rolle zu spielen.
MH Amino Acid Sequence; Animal; Base Sequence; Blotting, Southern; Blotting, Western; Brain/pathology; Molecular Sequence Data; Open Reading Frames; Polymerase Chain Reaction; Polymorphism, Restriction Fragment Length; Prion Diseases/genetics/*metabolism; Prions/*metabolism; Rats; Rats, Mutant Strains; Rats, Sprague-Dawley; Support, Non-U.S. Gov't
AD Institute for Virus Research, Kyoto University, Japan.
SP englisch
PO Irland