NR AERX
AU Goldmann,W.; Hunter,N.; Martin,T.; Dawson,M.; Hope,J.
TI Different forms of the bovine PrP gene have five or six copies of a short, G-C-rich element within the protein-coding exon
QU Journal of General Virology 1991 Jan; 72(1): 201-4
PT journal article
AB Current models of the virus-like agents of scrapie and bovine spongiform encephalopathy (BSE) have to take into account that structural changes in a host-encoded protein (PrP protein) exhibit an effect on the time course of these diseases and the survival time of any man or animal exposed to these pathogens. We report here the sequence of different forms of the bovine PrP gene which contain either five or six copies of a short, G-C-rich element which encodes the octapeptide Pro-His-Gly-Gly-Gly-Trp-Gly-Gln or its longer variants Pro-Gln/His-Gly-Gly-Gly-Gly-Trp-Gly-Gln. Out of 12 cattle, we found eight animals homozygous for genes with six copies of the Gly-rich peptide (6:6), while four were heterozygous (6:5). Two confirmed cases of BSE occurred in (6:6) homozygous animals.
IN Bei Kühen gibt es verschiedene Allele des PrP-Proteins, die sich in der Zahl der Kopien des Oktapeptides Pro-His-Gly-Gly-Gly-Trp-Gly-Gln oder des Nonapeptides Pro-Gln/His-Gly-Gly-Gly-Gly-Trp-Gly-Gln unterscheiden. Das offenbar häufigere Allel kodiert 6, das seltenere nur 4 Widerholungen des G/C-reichen Motives. Zwei an BSE gestorbene Rinder besaßen zwei Allele für die sechsfache Wiederholung.
MH Alleles; Amino Acid Sequence; Animal; Base Sequence; Cattle; DNA/blood/isolation & purification; DNA, Viral/isolation & purification; *Exons; Female; *Genes, Viral; Leukocytes/chemistry; Molecular Sequence Data; Oligonucleotide Probes; Polymerase Chain Reaction; PrPsc Proteins; Prions/*genetics; Viral Proteins/*genetics
AD AFRC Institute for Animal Health, Edinburgh, U.K.
SP englisch
PO England