NR AERW
AU Goldmann,W.; Hunter,N.; Benson,G.; Foster,J.D.; Hope,J.
TI Different scrapie-associated fibril proteins (PrP) are encoded by lines of sheep selected for different alleles of the Sip gene
QU Journal of General Virology 1991 Oct; 72(10): 2411-7
PT journal article
AB The incubation period of scrapie in sheep is controlled by the Sip gene which has two alleles (sA and pA). Following experimental challenge with SSBP/1 scrapie, a short incubation period is conferred by the partially dominant sA allele. Restriction fragment length polymorphisms of the scrapie-associated fibril protein (PrP) gene are associated with the Sip alleles. By sequencing the protein coding region of the PrP gene in Cheviot sheep selected for differing Sip genotypes, we have found four PrP protein variants which differ at three positions: amino acid 112 (Ala/Val), amino acid 130 (Arg/His) and amino acid 147 (Arg/Gln). The Val 112 variant can be distinguished at the DNA level by an RspXI restriction site which is not present in the Ala 112 form. Val 112 appears to be linked to a short incubation period of experimentally induced scrapie in the Cheviot sheep and therefore with the Sip sA allele. These results provide new evidence that the PrP protein may be a product of the Sip locus.
IN Die Inkubationszeit von Scrapie in Schafen hängt vom Sip-Gen ab, von dem es die beiden Allele sA und pA gibt. Das partiell dominante sA Allel bewirkt eine kurze Inkubationszeit von SSBP/1 Scrapie. Verschiedene Allele des möglicherweise das PrP-Protein kodierenden Sip-Gens unterscheiden sich durch Punktmutationen an den Positionen 112 (Ala/Val), 130 (Arg/His) und 147 (Arg/Gln). Die Val 112 Variante führt zu kurzen Inkubationzeiten und scheint dem Sip sA Allel zu entsprechen.
MH Alleles; Animal; Arginine/chemistry; Base Sequence; DNA, Viral; Genotype; Glycine/chemistry; Haplotypes; Molecular Sequence Data; Polymorphism, Restriction Fragment Length; PrPsc Proteins; Prions/*genetics; Restriction Mapping; Scrapie/*genetics; Sheep
AD Institute for Animal Health, AFRC & MRC Neuropathogenesis Unit, Edinburgh, U.K.
SP englisch
PO England