NR AEQP
AU Golaz,O.; Sanchez,J.C.; James,R.W.; Hochstrasser,D.F.
TI Phenotyping of apolipoprotein E using immobilized pH gradient gels for one-dimensional and two-dimensional separations
QU Electrophoresis 1995 Jul; 16(7): 1184-6
PT journal article
AB Apolipoprotein E (apo E) is a normal component of several classes of plasma lipoproteins. Apo E phenotypes are closely related to total cholesterol, low density lipoprotein (LDL)-cholesterol and apo B concentration. The apo E 2/2 phenotype is related to the type III hyperlipoproteinemia due to the defective binding of apo E-2 to the hepatic receptors. The apo E 4/4 phenotype has been reported to be present in most elderly people suffering from the Alzheimer disease, and is associated with increased risk of coronary heart disease and Creutzfeldt-Jakob disease. Therefore, apo E phenotyping is essential. The method described here uses a precast immobilized pH gradient, avoids time-consuming separation of lipoproteins from plasma, needs no pretreatment with neuraminidase and involves highly sensitive enhanced chemiluminescence for visualization. Therefore it has many advantages over previously published methods.
MH Apolipoproteins E/*genetics/isolation & purification; Electrophoresis, Gel, Two-Dimensional/*instrumentation; Electrophoresis, Polyacrylamide Gel/*instrumentation; Human; Hydrogen-Ion Concentration; Phenotype
AD Clinical Chemistry Laboratory, Geneva University Hospital, Switzerland.
SP englisch
PO Deutschland