NR AEMG
AU German,T.L.; McMillan,B.C.; Castle,B.E.; Dees,C.; Wade,W.F.; Marsh,R.F.
TI Comparison of RNA from healthy and scrapie-infected hamster brain
QU Journal of General Virology 1985 Apr; 66(4): 839-44
PT journal article
AB Density gradient fractions prepared from healthy or scrapie-infected hamster brain tissue enriched in plasma membrane vesicles were treated with nucleases prior to phenol extraction and ethanol precipitation. The recovered nucleic acids were 3' end-labelled and run on one-dimensional polyacrylamide gels. Autoradiography revealed the presence of low molecular weight RNAs (4S) in both healthy and scrapie samples. Two-dimensional fingerprint analysis indicated that the RNAs isolated from scrapie-infected hamsters contained oligonucleotides that were not present in RNAs isolated from healthy hamsters.
MH Animal; Base Sequence; *Brain Chemistry; Comparative Study; Hamsters; RNA/analysis; RNA, Viral/*analysis; Scrapie/*metabolism; Sheep; Support, Non-U.S. Gov't; Support, U.S. Gov't, P.H.S.; Virus Replication
SP englisch
PO England