NR AEEG
AU Fraser,H.; Brown,K.L.; Stewart,K.; McConnell,I.; McBride,P.A.; Williams,A.
TI Replication of scrapie in spleens of SCID mice follows reconstitution with wild-type mouse bone marrow
QU Journal of General Virology 1996 Aug; 77(8): 1935-40
PT journal article
AB SCID mice are resistant to intraperitoneal infection with 10(3) and 10(4) intracerebral ID50 units of ME7 scrapie agent whereas they develop disease after intracerebral challenge. However, higher doses introduced, by intraperitoneal or subcutaneous routes, produce disease. Immunocompetent mice of the same strain (CB20) developed scrapie following either intracerebral or intraperitoneal infection. Bioassay of spleens from SCID mice infected with 10(-1) dilutions of ME7 scrapie by intraperitoneal, intracerebral or abdominal subcutaneous injection showed traces or low levels of infectivity in spleen. However, subcutaneous injection beneath the skin of the neck failed to infect the spleen. CB20 bone marrow reconstitution of SCID mice resulted in the regeneration of a normal lymphoid architecture in the spleen. Spleens from these reconstituted mice, infected intracerebrally with a 10(-1) dilution of ME7 contained high levels of infectivity. These results suggest that the ability to replicate scrapie agent in spleen or lymphoid tissue depends on the restoration of normal lymphoid structure and in particular the presence of differentiated follicular dendritic cells. The possibility that SCID mice can select minor strains of scrapie which are normally unrecognized in cloned ME7 is discussed.
IN SCID-Mäuse benötigen für tödliche Scrapie-Infektionen bei Injektionen in die Bauchhöhle oder die Peripherie größere Erregermengen und enthalten nur geringe Erregerkonzentrationen in der Milz. Wird ihr Immunsystem durch Knochenmarktransplantationen repariert, dann verschwinden diese Unterschiede.
ZR 29
MH Animal; Animals, Wild; Biological Assay; Bone Marrow Transplantation; Dendritic Cells; Mice; Mice, Inbred BALB C; Mice, Inbred C3H; Mice, SCID; Prions/*biosynthesis/immunology; Scrapie/*etiology/immunology; *Spleen
AD Hugh Fraser (HughFraser@bbsrc.ac.uk), Karen L. Brown (karen.brown@bbsrc.ac.uk), Karen Stewart, Patricia A. McBride (tricia.mcbride@bbsrc.ac.uk), Institute for Animal Health, BBSRC and MRC, Neuropathogenesis Unit, Ogston Building, West Mains Road, Edinburgh EH9 3JF, Scotland, UK
SP englisch
PO England
OR Prion-Krankheiten 3