NR ADVW
AU Fairbairn,D.W.; Carnahan,K.G.; Thwaits,R.N.; Grigsby,R.V.; Holyoak,G.R.; O'Neill,K.L.
TI Detection of apoptosis induced DNA cleavage in scrapie-infected sheep brain
QU FEMS Microbiology Letters 1994 Jan 15; 115(2-3): 341-6
PT journal article
AB The pathogenesis and molecular basis of nerve cell death which accompanies scrapie infections in sheep are not well understood. Degeneration of neurons in culture caused by prion protein fragments has recently been reported to be consistent with mechanisms of cell death by apoptosis or programmed cell death. Apoptosis activation during prion-related encephalopathies has not yet been established in vivo. We report here the detection of DNA damage consistent with apoptosis in the brain cells of sheep infected with scrapie using laser scanning microscopic analysis of the single cell gel assay. We suggest that this DNA fragmentation is the result of the activation of the mechanisms characteristic of apoptotic cell death.
IN In Schafsgehirnen scheint die Scrapie-Infektion einen Mechanismus zu aktivieren, der die DNA von Nervenzellen fragmentiert.
MH Animal; *Apoptosis; Brain/*pathology; Cell Separation; *DNA Damage; Lasers; Microscopy/methods; Scrapie/*pathology; Sheep
AD Department of Microbiology, Brigham Young University, Provo, UT 84602.
SP englisch
PO Niederlande