NR ADQN
AU Duguid,J.R.; Rohwer,R.G.; Seed,B.
TI Isolation of cDNAs of scrapie-modulated RNAs by subtractive hybridization of a cDNA library
QU Proceedings of the National Academy of Sciences of the United States of America 1988 Aug; 85(15): 5738-42
PT journal article
AB We have developed a subtractive cloning procedure based on the hybridization of single-stranded cDNA libraries constructed in pi H3M, a vector containing the phage M13 origin of replication. We have used this strategy to isolate three transcripts whose abundance is increased in scrapie-infected brain. DNA sequence analysis showed that they represent glial fibrillary acidic protein, metallothionein II, and the B chain of alpha-crystallin; the latter two may represent a response to stress.
IN Als Reaktion auf eine Scrapie-Infektion scheint im Gehirn die Expression des sauren, glialen, fibrillären Proteins, des Metallothioneins II,und der beta-Kette des alpha-Crystallins zuzunehmen.
MH Amino Acid Sequence; Animal; Base Sequence; Brain Chemistry; Cloning, Molecular; Crystallins/genetics; DNA/genetics/*isolation & purification; Electrophoresis, Agar Gel; Female; Glial Fibrillary Acidic Protein/genetics; Hamsters; Mesocricetus; Metallothionein/genetics; Molecular Sequence Data; Nucleic Acid Hybridization; RNA/*genetics; Scrapie/*genetics; Support, Non-U.S. Gov't; Transcription, Genetic
AD GRECC, Edith Nourse Rogers Memorial Veterans Administration Hospital, Bedford, MA 01730.
SP englisch
PO USA