NR ADLP
AU Diringer,H.; Hilmert,H.; Simon,D.; Werner,E.; Ehlers,B.
TI Towards purification of the scrapie agent
QU European Journal of Biochemistry 1983 Aug 15; 134(3): 555-60
PT journal article
AB A method for the partial purification of scrapie infectivity from hamster brain is described. About a 100-1000-fold, 20-fold, and 200-fold enrichment in scrapie infectivity with respect to protein, RNA, and DNA content has been achieved using differential centrifugation, enzyme and detergent treatment. The inbred CLAC strain of hamsters used in our experiments contained about 10 times less infectivity in brain than has been found in randomly bred animals or other inbred strains.
MH Animal; Brain/microbiology; DNA, Viral/isolation & purification; Hamsters; Prions/analysis/*isolation & purification; RNA, Viral/isolation & purification; Scrapie/microbiology; Sheep; Support, Non-U.S. Gov't; Viral Proteins/isolation & purification
SP englisch
PO Westdeutschland