NR ADGX
AU Derrington,E.A.; Gabus,C.; Leblanc,P.; Chnaiderman,J.; Grave,L.; Dormont,D.; Swietnicki,W.; Morillas,M.; Marck,D.; Nandi,P.; Darlix,J.L.
TI PrPc has nucleic acid chaperoning properties similar to the nucleocapsid protein of HIV-1
QU Comptes Rendus de l'Academie des Sciences. Serie III, Sciences de la Vie 2002 Jan; 325(1): 17-23
PT journal article; review; review, tutorial
AB The function of the cellular prion protein (PrPc) remains obscure. Studies suggest that PrPc functions in several processes including signal transduction and Cu2+ metabolism. PrPc has also been established to bind nucleic acids. Therefore we investigated the properties of PrPc as a putative nucleic acid chaperone. Surprisingly, PrPc possesses all the nucleic acid chaperoning properties previously specific to retroviral nucleocapsid proteins. PrPc appears to be a molecular mimic of NCP7, the nucleocapsid protein of HIV-1. Thus PrPc, like NCP7, chaperones the annealing of tRNA(Lys) to the HIV-1 primer binding site, the initial step of retrovirus replication. PrPc also chaperones the two DNA strand transfers required for production of a complete proviral DNA with LTRs. Concerning the functions of NCP7 during budding, PrPc also mimices NCP7 by dimerizing the HIV-1 genomic RNA. These data are unprecedented because, although many cellular proteins have been identified as nucleic acid chaperones, none have the properties of retroviral nucleocapsid proteins.
ZR 15
MH Binding Sites; Capsid/*physiology; DNA, Single-Stranded/biosynthesis; DNA, Viral/biosynthesis; Dimerization; Gene Products, gag/*physiology; HIV-1/genetics; Molecular Chaperones/*physiology; Nucleic Acids/*metabolism; PrPc Proteins/*physiology; RNA, Transfer, Lys/metabolism; RNA, Viral/chemistry; Support, Non-U.S. Gov't; Transcription, Genetic; Virus Replication
AD Edmund Derrington, Caroline Gabus, Pascal Leblanc, Jonas Chnaidermann, Linda Grave, Jean-Luc Darlix, Laboretro, unité de virologie humaine, Inserm-ENS U412, ENS de Lyon, 46, allée d?Italie, 69364 Lyon, France; Dominique Dormont, Département de Recherche Médicale, CEA, BP 6, 92265 Fontenay-aux-Roses, France; Wieslaw Swietnicki, Manuel Morillas, Department of Pathology, Case Western Reserve University, Cleveland, Ohio 44106, USA; Daniel Marck, Pradip Nandi, Inra, centre de recherche de Tours, 37380 Nouzilly, France
SP englisch
PO Frankreich