NR ADGR
AU Derkatch,I.L.; Bradley,M.E.; Zhou,P.; Liebman,S.W.
TI The PNM2 mutation in the prion protein domain of SUP35 has distinct effects on different variants of the [PSI+] prion in yeast
QU Current Genetics 1999 Mar; 35(2): 59-67
PT journal article
AB We have previously described different variants of the yeast prion [PSI+] that can be obtained and maintained in the same genetic background. These [PSI+] variants, which differ in the efficiency of nonsense suppression, mitotic stability and the efficiency of curing by GuHCl, may correspond to different [PSI+] prion conformations of Sup35p or to different types of prion aggregates. Here we investigate the effects of overexpressing a mutant allele of SUP35 and find different effects on weak and strong [PSI+] variants: the suppressor phenotype of weak [PSI+] factors is increased, whereas the suppressor effect of strong [PSI+] factors is reduced. The SUP35 mutation used was originally described as a "Psi no more" mutation (PNM2) because it caused loss of [PSI+]. However, none of the [PSI+] variants in the strains used in our study were cured by PNM2. Indeed, when overexpressed, PNM2 induced the de novo appearance of both weak and strong [PSI+] variants with approximately the same efficiency as the overexpressed wild-type SUP35 allele. Our data suggest that the change in the region of oligopeptide repeats in the Sup35p N-terminus due to the PNM2 mutation modifies, but does not impair, the function of the prion domain of Sup35p.
MH Alleles; Binding Sites; Fungal Proteins/chemistry/*genetics/metabolism; Gene Expression Regulation; Gene Expression Regulation, Fungal; Luminescent Proteins/genetics; Mutation; Prions/*genetics/metabolism; Protein Structure, Tertiary; Recombinant Fusion Proteins/genetics/metabolism; Saccharomyces cerevisiae/*genetics/metabolism; Support, Non-U.S. Gov't; Support, U.S. Gov't, P.H.S.; Variation (Genetics)
AD Laboratory for Molecular Biology, Department of Biological Sciences, University of Illinois at Chicago, 900 S. Ashland Avenue, Chicago, IL 60304, USA
SP englisch
PO USA