NR ADFY
AU Demaimay,R.; Chesebro,B.; Caughey,B.W.
TI Inhibition of formation of protease-resistant prion protein by Trypan Blue, Sirius Red and other Congo Red analogs
QU Archives of Virology. Supplementum 2000(16): 277-83
PT journal article
AB Five compounds related to Congo Red were found to inhibit generation of protease-resistant prion protein in a cell-free system. In this assay Trypan Blue, Evans Blue, Sirius Red F3B, Primuline and Thioflavin-S were all more inhibitory than Congo Red itself. In scrapie-infected mouse neuroblastoma cells one compound, Sirius Red F3B, was capable of blocking the formation of protease-resistant prion protein to a similar extent as Congo Red; however, the other four compounds were less effective. Some of these compounds should be considered for testing in TSE disease models in live animals.
MH Animal; Azo Compounds/pharmacology; Blotting, Western; Cell-Free System; Congo Red/*analogs & derivatives/*pharmacology; Hamsters; Mice; Neuroblastoma; Neurons/chemistry; PrPc Proteins/chemistry/*drug effects/metabolism; PrPsc Proteins/*chemistry/*drug effects/metabolism; Scrapie/metabolism; Thiazoles/pharmacology; Trypan Blue/*pharmacology; Tumor Cells, Cultured
AD RML, NIAID, NIH, Hamilton, Montana 59840, USA
SP englisch
PO Österreich