NR ACZH
AU Cutlip,R.C.; Miller,J.M.; Race,R.E.; Jenny,A.L.; Katz,J.B.; Lehmkuhl,H.D.; DeBey,B.M.; Robinson,M.M.
TI Intracerebral transmission of scrapie to cattle
QU Journal of Infectious Diseases 1994 Apr; 169(4): 814-20
PT journal article
AB To determine if sheep scrapie agent(s) in the United States would induce a disease in cattle resembling bovine spongiform encephalopathy, 18 newborn calves were inoculated intracerebrally with a pooled suspension of brain from 9 sheep with scrapie. Half of the calves were euthanatized 1 year after inoculation. All calves kept longer than 1 year became severely lethargic and demonstrated clinical signs of motor neuron dysfunction that were manifest as progressive stiffness, posterior paresis, general weakness, and permanent recumbency. The incubation period was 14-18 months, and the clinical course was 1-5 months. The brain from each calf was examined for lesions and for protease-resistant prion protein. Lesions were subtle, but a disease-specific isoform of the prion protein was present in the brain of all calves. Neither signs nor lesions were characteristic of those for bovine spongiform encephalopathy.
IN Ein Hirngemisch aus 6 in den USA an Scrapie erkrankten Schafen wurde 18 neugeborenen Kälbern in die Gehirne injiziert. Die Hälfte der Kälber wurde nach 1 Jahr getötet und zeigte bereits subtile Gehirnschäden sowie die krankheitsspezifische Form des Prionproteins. Die andere Hälfte der Kälber zeigte nach 14-18 Monaten die ersten Symptome und starb 1-5 Monate später.
MH Animal; Brain/*microbiology/pathology; Cattle; Cattle Diseases/*etiology/pathology; Encephalopathy, Bovine Spongiform/*etiology/pathology; Immunoblotting/veterinary; Immunohistochemistry; Male; Motor Neurons/physiology; Prions/analysis; Scrapie/pathology/*transmission; Sheep; Sleep Stages; Time Factors
AD USDA, Agriculture Research Service, National Animal Disease Center, Ames, IA 50010.
SP englisch
PO USA