NR ACOO
AU Chesebro,B.; Race,R.E.; Wehrly,K.; Nishio,J.; Bloom,M.E.; Lechner,D.; Bergstrom,S.; Robbins,K.; Mayer,L.; Keith,J.M.; Garon,C.; Haase,A.
TI Identification of scrapie prion protein-specific mRNA in scrapie-infected and uninfected brain
QU Nature 1985 May 23-29; 315(6017): 331-3
PT journal article
AB To date no nucleic acid has been found in the purified infectious agent which causes the spongiform encephalopathy known as scrapie. In an attempt to identify a unique scrapie virus-associated messenger RNA in tissues of infected animals, we have synthesized an oligonucleotide probe complementary to the mRNA sequence corresponding to the amino-acid sequence of the prion protein, PrP27-30 (ref. 1). We report here that, with this probe, a complementary DNA clone representing PrP27-30 was obtained from scrapie-infected mouse brain; the DNA sequence of this clone could be translated into a protein that matches exactly the published sequence of PrP27-30. The cDNA clone hybridized to a single 2.4-2.5-kilobase (kb) mRNA from both normal and scrapie-infected brain. Thus, the PrP27-30 mRNA is not uniquely associated with scrapie infectivity, suggesting that PrP27-30 may be a normal component of mouse and hamster brain.
MH Amino Acid Sequence; Animal; Base Sequence; Brain/*microbiology/physiology; Hamsters; Mice; Prions/*genetics; RNA, Messenger/*genetics/isolation & purification; Viral Proteins/*genetics
SP englisch
PO England