NR ACNW
AU Chen,S.G.; Teplow,D.B.; Parchi,P.; Teller,J.K.; Gambetti,P.; Autilio-Gambetti,L.
TI Truncated forms of the human prion protein in normal brain and in prion diseases
QU The Journal of Biological Chemistry 1995 Aug 11; 270(32): 19173-80
PT journal article
AB The cellular form of the prion protein (PrPc) is a glycoprotein anchored to the cell membrane by a glycosylphosphatidylinositol moiety. An aberrant form of PrPc that is partially resistant to proteases, PrPres, is a hallmark of prion diseases, which in humans include Cruetzfeldt-Jakob disease (CJD), Gerstmann-Sträussler-Scheinker syndrome, and fatal familial insomnia. We have characterized the major forms of PrP in normal and pathological human brains. A COOH-terminal fragment of PrPc, designated C1, is abundant in normal and CJD brains as well as in human neuroblastoma cells. Sequence analysis revealed that C1 contains alternative NH2 termini starting at His-111 or Met-112. Like PrPc, C1 is glycosylated, anchored to the cell membrane, and is heat-stable. Consistent with the lack of the NH2-terminal region of PrPc, C1 is more acidic than PrPc and does not bind heparin. An additional fragment longer than C1, designated C2, is present in substantial amounts in CJD brains. Like PrPres, C2 is resistant to proteases and is detergent-insoluble. Our data indicate that C1 is a major product of normal PrPc metabolism, generated by a cleavage that disrupts the neurotoxic and amyloidogenic region of PrP comprising residues 106-126. This region remains intact in C2, suggesting a role for C2 in prion diseases.
IN Normale ebenso wie die Gehirne von Creutzfeldt Jakob-Kranken enthalten größere Mengen eines Prionproteinfragmentes, dem die ersten aminoterminalen 110 oder 111 Aminosäuren fehlen. Es ist glykosyliert, in der Zellmembran verankert und hitzestabil. Es ist aber saurer als das vollständige Prionprotein und bindet nicht an Heparin. Die Gehirne von Creutzfeldt Jakob-Kranken enthalten außerdem ein größeres Prionproteinfragment, dem die neurotoxische und amylogene Region 106-126 nicht fehlt. Diese Form ist proteaseresistent und in Detergens unlöslich.
ZR 60
MH Adult; Aged; Aged, 80 and over; Amino Acid Sequence; *Brain Chemistry; Human; Middle Age; Molecular Sequence Data; Neuroblastoma/chemistry; Peptide Fragments/analysis; Prion Diseases/*metabolism; Prions/*analysis/chemistry; Support, U.S. Gov't, P.H.S.; Tumor Cells, Cultured
AD Division of Neuropathology, Case Western Reserve University, Cleveland, Ohio 44106, USA
SP englisch
PO USA
OR Prion-Krankheiten C