NR ABZU
AU Büeler,H.; Fischer,M.; Lang,Y.; Bluethmann,H.; Lipp,H.P.; DeArmond,S.J.; Prusiner,S.B.; Aguet,M.; Weissmann,C.
TI Normal development and behaviour of mice lacking the neuronal cell-surface PrP protein
QU Nature 1992 Apr 16; 356(6370): 577-82
KI Nature. 1992 Apr 16;356(6370):560. PMID: 1348569
PT journal article
AB PrPc is a host protein anchored to the outer surface of neurons and to a lesser extent of lymphocytes and other cells. The transmissible agent (prion) responsible for scrapie is believed to be a modified form of PrPc. Mice homozygous for disrupted PrP genes have been generated. Surprisingly, they develop and behave normally for at least seven months, and no immunological defects are apparent. It is now feasible to determine whether mice devoid of PrPc can propagate prions and are susceptible to scrapie pathogenesis.
IN Es wurden homozygote Mäuse mit funktionsunfähigen Prionproteingenen erzeugt, die während der ersten 7 Monate ihres Lebens keine Auffälligkeiten erkennen ließen.
MH Aging; Animal; Base Sequence; Blastocyst/physiology; Blotting, Northern; Blotting, Western; Brain/growth & development/*physiology; Cell Membrane/physiology; Crosses, Genetic; Female; Heterozygote Detection; Male; Membrane Proteins/*genetics; Mice; Molecular Sequence Data; Neurons/*physiology; Oligodeoxyribonucleotides; Polymerase Chain Reaction; PrPsc Proteins; Pregnancy; Prions/*genetics; RNA/genetics/isolation & purification; Recombination, Genetic; Restriction Mapping; Scrapie/*genetics; Stem Cells/physiology; Support, Non-U.S. Gov't; Support, U.S. Gov't, P.H.S.
AD Prof. Charles Weissmann, Universität Zürich, Institut für Molekularbiologie, Abteilung I, Hönggerberg, 8093 Zürich, Schweiz, Postfach, Tel. 633 24 91, Fax 371 72 05
SP englisch
PO England
OR Prion-Krankheiten 2