NR ABWF
AU Brown,P.; Coker-Vann,M.; Pomeroy,K.; Franko,M.; Asher,D.M.; Gibbs,C.J.Jr.; Gajdusek,D.C.
TI Diagnosis of Creutzfeldt-Jakob disease by Western blot identification of marker protein in human brain tissue
QU The New England Journal of Medicine 1986 Feb 27; 314(9): 547-51
PT journal article
AB We tested purified preparations of brain tissue from 39 patients with Creutzfeldt-Jakob disease, Gerstmann-Sträussler-Scheinker syndrome, or kuru, and from 32 patients with a variety of nonspongiform degenerative diseases, with the use of Western blots against an antiserum to a similarly purified fraction made from scrapie-infected hamster brain. Positive reactions occurred in 81 percent of the 31 specimens from the patients with Creutzfeldt-Jakob disease (and in all of the 7 specimens that were stored frozen for less than one year), in 3 of the 4 specimens from the patients with kuru, in 3 of the 4 specimens from the patients with Gerstmann-Sträussler-Scheinker syndrome, and in none of the specimens from the patients with other neurologic degenerative disorders, including familial or sporadic Alzheimer's disease; dementia associated with myoclonus, motor neuron disease, or parkinsonism; and acquired-immunodeficiency-syndrome encephalopathy. Immunologic testing has thus begun to provide a useful and rapid adjunct to neuropathological examinations and animal-transmission experiments for the diagnosis of the spongiform encephalopathies.
MH Animal; *Brain Chemistry; Brain Diseases/diagnosis; Creutzfeldt-Jakob Syndrome/*diagnosis; Hamsters; Human; Immunoelectrophoresis; Kuru/diagnosis; Microscopy, Electron; Nerve Tissue Proteins/immunology/*isolation & purification; Neurofibrils/analysis/immunology; Rabbits; Scrapie/immunology
AD Paul Brown (pwb@codon.nih.gov), Laboratory of Central Nervous System Studies, National Institute of Neurological and Communicative Disorders and Stroke, National Institutes of Health, Bethesda, Maryland 20892.
SP englisch
PO USA