NR ABTH
AU Brown,D.R.; Herms,J.W.; Kretzschmar,H.A.
TI Mouse cortical cells lacking cellular PrP survive in culture with a neurotoxic PrP fragment
QU Neuroreport 1994 Oct 27; 5(16): 2057-60
PT journal article
AB To elucidate whether the neurotoxic effect of a prion protein fragment (PrP106-126) is in some way mediated by the cellular isoform of the prion protein (PrPc), dissociated cortical cell cultures were prepared from mice in which the PrP gene had been disrupted (PrP0/0 mice). Cell survival after 10 days in culture was tested with an MTT assay. PrP106-126 applied every second day for 10 days in cultures from normal mice resulted in the death of 34% more cells than in untreated cells. When PrP106-126 was applied to cultures from mice lacking PrPc expression, survival was equal to or greater than that of untreated control cells. These results support the notion that expression of PrPc is required for the neurotoxic effect of PrP106-126.
IN Hirnrindenzellkulturen aus Mäusen ohne zelluläre Prionproteine sind immun gegen neurotoxische Peptide, die der Prionproteinsequenz 106-126 entsprechen. Auch die neurotoxische Wirkung der Peptide wird also über das normale zelluläre Prionprotein vermittelt.
MH Amino Acid Sequence; Animal; Cell Survival/physiology; Cells, Cultured; Cerebral Cortex/cytology/*drug effects; Mice; Mice, Inbred C57BL; Molecular Sequence Data; Peptide Fragments/*toxicity; Prions/*analysis/*toxicity; Support, Non-U.S. Gov't
AD Department of Neuropathology, University of Göttingen, Germany.
SP englisch
PO England