NR ABMP
AU Borchelt,D.R.; Koliatsos,V.E.; Guarnieri,M.; Pardo,C.A.; Sisodia,S.S.; Price,D.L.
TI Rapid anterograde axonal transport of the cellular prion glycoprotein in the peripheral and central nervous systems
QU The Journal of Biological Chemistry 1994 May 20; 269(20): 14711-4
IA http://www.jbc.org/cgi/reprint/269/20/14711
PT journal article
AB In prion diseases, the cellular prion protein (PrPc), abundant in neurons, is converted posttranslationally into an amyloid-forming scrapie prion protein (PrPsc), which accumulates in white matter tracts and nerve terminals. The trafficking of PrPc in neurons was investigated in vivo by injecting [35S]methionine into the L4 and L5 dorsal root ganglia and the entorhinal cortices of adult rats and by tracing the movement of radiolabeled PrPc. In both paradigms, labeled 33-35-kDa PrPc was transported, within 4 h, to distal axons and nerve terminals cofractionating with proteins in the fast component. Future studies using these methods may allow us to determine whether PrPc is converted into PrPsc during axonal transport and whether PrPsc is transported in animals with prion diseases.
IN Durch radioaktive Markierung konnte gezeigt werden, dass das normale Prionprotein bei Ratten aus dem zentralen Zellkörper relativ schnell in die Enden der Nervenfasern transportiert wird.
MH Animals; Axonal Transport; Brain/metabolism/*physiology; Cell Line; Cricetinae; Electrophoresis, Polyacrylamide Gel; Ganglia, Spinal/metabolism/*physiology; Mesocricetus; Methionine/metabolism; Nerve Endings/metabolism/physiology; Nerve Tissue Proteins/*metabolism; Neurons/metabolism/*physiology; Organ Specificity; PrPsc Proteins; Prions/biosynthesis/isolation & purification/*metabolism; Rabbits/immunology; Rats; Rats, Sprague-Dawley; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.; Sciatic Nerve/metabolism/*physiology; Sulfur Radioisotopes
AD Department of Pathology, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205-2196.
SP englisch
PO USA