NR ABJL
AU Birkett,C.R.; Hennion,R.M.; Bembridge,D.A.; Clarke,M.C.; Chree,A.; Bruce,M.E.; Bostock,C.J.
TI Scrapie strains maintain biological phenotypes on propagation in a cell line in culture
QU EMBO Journal 2001 Jul 2; 20(13): 3351-8
IA http://www.pubmedcentral.gov/articlerender.fcgi?tool=pubmed&pubmedid=11432823
PT journal article
AB Bovine spongiform encephalopathy (BSE) and its human equivalent, variant Creutzfeldt-Jakob disease (vCJD), are caused by the same strain of infectious agent, which is similar to, but distinct from, >20 strains of their sheep scrapie homologue. A better understanding of the molecular strain determinants could be obtained from cells in monoculture than from whole animal studies where different cell targeting is commonly a strain-related feature. Although a few cell types can be infected with different strains, the phenotypes of the emergent strains have not been studied. We have cured the scrapie-infected, clonal SMB cell line with pentosan sulfate, stably re-infected it with a different strain of scrapie and shown that biological properties and prion protein profiles characteristic of each original strain are propagated faithfully in this single non-neuronal cell type. These findings attest to the fact that scrapie strain determinants are stable and host-independent in isolated cells.
MH Animals; Brain/metabolism; Cattle; Cell Line; Creutzfeldt-Jakob Syndrome/etiology; Encephalopathy, Bovine Spongiform/etiology; Humans; Mice; Phenotype; PrPsc Proteins/biosynthesis/*genetics; Protein Isoforms/biosynthesis/genetics/physiology; Research Support, Non-U.S. Gov't; Scrapie/*etiology/physiopathology
AD Christopher R. Birkett (chris.birkett@bbsrc.ac.uk), Ruth M. Hennion, Michael C. Clarke, Christopher J. Bostock, Institute for Animal Health, Compton Laboratory, Compton, Newbury, RG20 7NN, UK; Aileen Chree, Moira E. Bruce (moira.bruce@bbsrc.ac.uk), Institute for Animal Health, Neuropathogenesis Unit, Ogston Building, West Mains Road, Edinburgh EH9 3JF, UK
SP englisch
PO England
OR Prion-Krankheiten 2