NR ABFM
AU Bencsik,A.A.; Lezmi,S.; Hunsmann,G.; Baron,T.G.M.
TI Close vicinity of PrP expressing cells (FDC) with noradrenergic fibers in healthy sheep spleen
QU Developmental Immunology 2001; 8(3-4): 235-41
PT journal article
AB In naturally and experimentally occurring scrapie in sheep, prions invade the immune system and replicate in lymphoid organs. Here we analysed immunohistochemically, in seven spleens of 6-month-old healthy sheep, the nature of the cells expressing prion protein (PrP) potentially supporting prion replication, as well as their relationship with autonomic innervation. PrP was identified using either RB1 rabbit antiserum or 4F2 monoclonal antibody directed against AA 108-123 portion of the bovine and AA 79-92 of human prion protein respectively. Using double labelling analysis, we demonstrated that PrPc is expressed by follicular dendritic cells using a specific monoclonal antibody (CNA42). We also showed the close vicinity of these PrP expressing cells with noradrenergic fibers, using a polyclonal tyrosine hydroxylase antibody. Our results may help the study of the cellular requirements for the possible neuroinvasion from the spleen.
MH Adrenergic Fibers/*chemistry; Animal; Dendritic Cells, Follicular/*metabolism; Immunohistochemistry; Norepinephrine/analysis; Prions/immunology/*metabolism; Sheep/anatomy & histology/immunology/*metabolism; Spleen/cytology/*innervation/*metabolism; Support, Non-U.S. Gov't
AD Anna A. Bencsik (a.bencsik@lyon.afssa.fr), Stéphane Lezmi (s.lezmi@lyon.afssa.fr), Thierry G. M. Baron (t.baron@lyon.afssa.fr), Unité Agents Transmissibles Non Conventionnels (ATNC), Agence Francaise de Sécurité Sanitaire des Aliments (AFSSA), Laboratoire d'Etudes et de Recherches en Pathologie Bovine et Hygiène des Viandes, 31 Avenue Tony Garnier, 69364 Lyon cedex 07, France; Gerhard Hunsmann, Department of Virology and Immunology, Kellnerweg 4, 37077 Göttingen, Germany
SP englisch
PO England