NR ABCF
AU Baxter,H.C.; Fraser,J.R.; Liu,W.G.; Forster,J.L.; Clokie,S.; Steinacker,P.; Otto,M.; Bahn,E.; Wiltfang,J.; Aitken,A.
TI Specific 14-3-3 isoform detection and immunolocalization in prion diseases
QU Biochemical Society Transactions 2002 Aug; 30(4): 387-91
PT journal article
AB 14-3-3 proteins are involved in signalling processes in neuronal cells. Using isoform-specific antibodies we have examined the variation in 14-3-3 isoform neurolocation in normal and scrapie-infected murine brain and show that in defined areas of the brain there are significant changes associated with the pathology of the disease process. The appearance of 14-3-3 proteins in the cerebrospinal fluid (CSF) is a consequence of neuronal disease and the detection of specific isoforms of the 14-3-3 proteins in the CSF is characteristic of some neurodegenerative diseases. In this study, monitoring specifically for the gamma 14-3-3 isoform in the CSF by both Western-blot analysis and ELISA we can show a level of correlation between the assays.
AD H. C. Baxter (hbaxter@ed.ac.uk), J. L. Forster, S. Clokie, A. Aitken, Department of Biomedical Sciences, University of Edinburgh, George Square, Edinburgh EH8 9XD, U.K.; Janet R. Fraser (janet.fraser@bbsrc.ac.uk), Wing-Gee Liu (winggee.liu@bbsrc.ac.uk), Institute for Animal Health, BBSRC and MRC, Neuropathogenesis Unit, Ogston Building, West Mains Road, Edinburgh EH9 3JF, Scotland, UK; P. Steinacker, M. Otto, E. Bahn, J. Wiltfang, Departments of Neurology, Neuropathology and Psychiatry, University of Göttingen, D-37075, Göttingen, Germany
SP englisch
PO England