NR ABBA
AU Basler,K.; Oesch,B.; Scott,M.R.D.; Westaway,D.; Walchli,M.; Groth,D.F.; McKinley,M.P.; Prusiner,S.B.; Weissmann,C.
TI Scrapie and cellular PrP isoforms are encoded by the same chromosomal gene
QU Cell 1986 Aug 1; 46(3): 417-28
PT journal article
AB PrP 27-30 is the major protein in purified preparations of scrapie agent. An almost complete PrP cDNA was used to select PrP-related genomic clones from normal hamster DNA. The gene contains a noncoding exon of 56 to 82 bp and a 2 kb coding exon, separated by a 10 kb intron. Transcription initiates at the same multiple sites in vivo and in vitro. The promoter lacks a TATA box and contains three repeats of the sequence GCCCCGCCC, which resembles the Sp1 binding site found in "housekeeping" genes. The PrP coding sequence encodes a presumptive amino-terminal signal peptide. The primary structure of PrP encoded by the gene of a healthy animal does not differ from that encoded by a cDNA from a scrapie-infected animal, suggesting that the different properties of PrP from normal and scrapie-infected brains are due to post-translational events.
IN Das Gen des Hamsterprionproteins besitzt ein nichtkodierendes 56-82 bp Exon und getrennt davon durch ein 10 kb Intron ein 2 kb Exon mit der kodierenden Region. Die Transkription beginnt in vitro und in vivo an verschiedenen Startpositionen. Der Promoter besitzt keine TATA-Box, jedoch 3 Wiederholungen der Sequenz GCCCCGCCC, welche der für Haushaltsgene typischen Sp1-Bindungsstelle ähnelt. Die kodierende Region kodiert ein Signalpeptid. Die untersuchten Prionprotein-mRNAs in gesunden und scrapieinfizierten Tieren.
MH Animal; Base Sequence; Brain Chemistry; Comparative Study; DNA/analysis; Hamsters/*genetics; Mesocricetus/*genetics; Poly A/genetics; PrP 27-30 Protein; Prions/*genetics; Promoter Regions (Genetics); Protein Precursors/biosynthesis; Protein Processing, Post-Translational; Protein Sorting Signals/genetics; RNA, Messenger/genetics; Support, Non-U.S. Gov't; Support, U.S. Gov't, P.H.S.; Viral Proteins/biosynthesis
AD Prof. Charles Weissmann, Institut für Molekularbiologie, Abteilung I, 8093 Zürich, Hönggerberg, Postfach, Tel. 6332491, Fax 3717205
SP englisch
PO USA