NR ABAD
AU Baron,T.G.M.; Madec,J.Y.; Calavas,D.; Richard,Y.; Barillet,F.
TI Comparison of French natural scrapie isolates with bovine spongiform encephalopathy and experimental scrapie infected sheep
QU Neuroscience Letters 2000 Apr 28; 284(3): 175-8
PT journal article
AB We compared the glycoform pattern of the abnormal prion protein (PrPsc) detected by immunoblotting in 21 sheep with natural scrapie, from 21 different outbreaks identified in France since 1996, with a bovine spongiform encephalopathy (BSE)-infected sheep. All the natural scrapie isolates had a higher molecular mass of the unglycosylated PrPsc than in BSE-infected sheep. In the latter case, this molecular mass appeared identical to that found in the CH 1641 experimental scrapie strain (type C pattern), whereas in natural scrapie cases it was similar to that found in the SSBP/1 experimental scrapie strains. These results suggest that all French natural scrapie isolates studied so far would belong, as SSBP/1, to the group of scrapie cases with type A electrophoretic pattern.
MH Animal; Blotting, Western; Cattle; Comparative Study; Electrophoresis, Polyacrylamide Gel; Encephalopathy, Bovine Spongiform/*metabolism; France/epidemiology; Glycosylation; Molecular Weight; PrPsc Proteins/*chemistry/classification/*isolation & purification; Scrapie/epidemiology/*metabolism; Sheep; Support, Non-U.S. Gov't
AD Agence Francaise de Securite Sanitaire des Aliments, 69364, Lyon, France. t.baron@lyon.afssa.fr
SP englisch
PO Irland