NR AAZS
AU Baroli,I.; Niyogi,K.K.
TI Molecular genetics of xanthophyll-dependent photoprotection in green algae and plants
QU Philosophical Transactions of the Royal Society of London. Series B: Biological Sciences 2000 Oct 29; 355(1402): 1385-94
IA http://www.journals.royalsoc.ac.uk/media/2fa1689wrk6qyjb7tpq0/contributions/q/p/j/a/qpjabmqk0kxar5vb.pdf
PT journal article; review; review, tutorial
AB The involvement of excited and highly reactive intermediates in oxygenic photosynthesis inevitably results in the generation of reactive oxygen species. To protect the photosynthetic apparatus from oxidative damage, xanthophyll pigments are involved in the quenching of excited chlorophyll and reactive oxygen species, namely 1Chl*, 3Chl*, and 1O2*. Quenching of 1Chl* results in harmless dissipation of excitation energy as heat and is measured as non-photochemical quenching (NPQ) of chlorophyll fluorescence. The multiple roles of xanthophylls in photoprotection are being addressed by characterizing mutants of Chlarnydomonas reinhardtii and Arabidopsis thaliana. Analysis of Arabidopsis mutants that are defective in 1Chl* quenching has shown that, in addition to specific xanthophylls, the psbS gene is necessary for NPQ. Double mutants of Chlamydomonas and Arabidopsis that are deficient in zeaxanthin, lutein and NPQ undergo photo-oxidative bleaching in high light. Extragenic suppressors of the Chlamydomonas npq1 lor1 double mutant identify new mutations that restore varying levels of zeaxanthin accumulation and allow survival in high light.
ZR 75
MH Algae, Green/metabolism; Animals; Arabidopsis; Chlamydomonas reinhardtii; Lutein/genetics/*metabolism; Oxidative Stress; Oxidoreductases/genetics/metabolism; Photosynthesis/*physiology; PrPc Proteins/metabolism; Reactive Oxygen Species; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S.; beta Carotene/analogs & derivatives/physiology
AD Department of Plant and Microbial Biology, University of California, Berkeley 94720-3102, USA
SP englisch
PO England